Allows you to design synthetic siRNA, Stealth RNA, or shRNA molecules from nucleotide target sequences, or convert an siRNA molecule sequence into a Stealth?molecule or shRNA molecule. It uses its proprietary algorithm or Tuschl's rules for siRNA design, - [Read more BLOCK-iT RNAi Designer]
Promega SiRNA Designer Tool. This program does not predict hairpins, but the 'split' technology developed by Rossi and colleagues. Like several other programs listed below, a useful aspect of the program is that the user may click on the displayed siRNAs - [Read more Promega SiRNA Designer Tool]
This tool generates hairpin siRNA-encoding DNA oligonucleotide inserts from an input siRNA target sequence. The program will add the loop sequence and overhangs for cloning as outlined in the vectors' Instruction Manuals. Ambion - [Read more pSilencer Converter Ambion]
RNAi Database. This database contains phenotypic data from RNA interference studies in C. elegans. Enter RNAiDB using one of the search options below - [Read more RNAi Database for C.Elegans]
This tool generates template DNA oligonucleotide sequences from an input siRNA target sequence. Designs for both the "two-oligonucleotide" and "single-oligonucleotide" approach are displayed. These designs are generated using the loop, terminator and RNA - [Read more Silencerâ„¢ Express siRNA Expression Cassette Kits PCR Primer Design Tool]
SiRNA at Whitehead. This site helps you select siRNAs to knock down your gene of interest. After entering your sequence or accession/gi number, choosing pattern for your oligo nucleotides and various filter methods, you will be presented with a list of ol - [Read more SiRNA at Whitehead]
The siRNA design tool scans a target gene for candidate siRNA sequences that satisfy user-adjustable rules. Selected candidates are then screened to identify those siRNA sequences that are specific to the gene of interest. Enter your gene, Gi or accessio - [Read more SiRNA Selector]
Find siRNA target sites in your mRNA of interest using the published recommendations of Tuschl and colleagues for siRNA design. Once identified, siRNA targets can be sent directly to one of the kit-specific design tools described below or subjected to a - [Read more siRNA Target Finder. Ambion.]
siSearch is designed to select siRNAs based on approaches derived from data mining of our siRNA database. We also allow common methods of siRNA design to be included in the search. This includes motif rules, energy conditions and specificity searching. In - [Read more SiSearch]
The human siRNA database. HuSiDa is a public database that serves as a depository for both, sequences of published functional siRNA molecules targeting human genes and important technical details of the corresponding gene silencing experiments. It aims at - [Read more The human siRNA database]
The RNAi Consortium shRNA Library. Short hairpin RNA (shRNA) clones produced by the TRC, as well as protocols for handling and conducting screens with shRNA molecules. The RNAi consortium shRNA library is distributed as bacterial glycerol stocks, plasmid - [Read more The RNAi Consortium shRNA Library]
